Functionalized MWCNTs are used in many commercial and biomedical applications, but their potential health effects are not\nwell defined. We investigated and compared cytotoxic, genotoxic/oxidative, and inflammatory effects of pristine and carboxyl\nMWCNTs exposing human respiratory (A549 and BEAS-2B) cells to 1ââ?¬â??40 ?g/mL of CNTs for 24 h. Both MWCNTs induced low\nviability reduction (by WST1 assay) in A549 cells and only MWCNTs-COOH caused high viability reduction in BEAS-2B cells\nreaching 28.5% viability at 40 ?g/mL. Both CNTs induced membrane damage (by LDH assay) with higher effects in BEAS-2B\ncells at the highest concentrations reaching 20% cytotoxicity at 40 ?g/mL. DNA damage (by Fpg-comet assay) was induced by\npristine MWCNTs in A549 cells and by both MWCNTs in BEAS-2B cells reaching for MWCNTs-COOH a tail moment of 22.2\nat 40 ?g/mL versus 10.2 of unexposed cells. Increases of IL-6 and IL-8 release (by ELISA) were detected in A549 cells exposed\nto MWCNTs-COOH from 10 ?g/mL while IL-8 increased in BEAS-2B cells exposed to pristine MWCNTs at 20 and 40 ?g/mL.\nThe results show higher cytogenotoxicity of MWCNTs-COOH in bronchial and of pristine MWCNTs in alveolar cells. Different\ninflammatory response was also found. The findings suggest the use of in vitro models with different end points and cells to study\nCNT toxicity.
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